Dr. Řádek

AnxoProtect Tbl

Food Supplement with Herbal Extracts

Packaging:

Dose 100 tablets

Composition:

Composition of 1 tablet 500mg: Ashwagandha - Withania somnifera extr.100mg, Holy Basil - Ocimum sanctum extr.100mg, Passion Flower- Passiflora incarnata extr.50mg, Gotu Kola - Centella asiatica extr.50mg, Sorbitol, Brahmi - Bacopa monieri extr.30mg, Guduchi - Tinospora cordifolia extr.30mg, Asparagus -Shatavari - Asparagus racemosus extr.30mg, Indian Gooseberry - Emblica officinalis extr.20mg, Magnesium stearate, Polydextrose, Microcrystalline cellulose,xylitol
One tablet contains: Stand.extracts Withania somnifera extr.100mg,Ocimum sanctum extr.100mg,Passiflora incarnata extr.50mg,Centella asiatica extr.50mg,Bacopa monieri extr.30mg,Tinospora cordifolia extr.30mg,Asparagus racemosus extr.30mg,Emblica officinalis extr.20mg

Recommended Dosage:

Adults: 2 times a day 1 - 2 tablets
Children from 15: Twice a day 1 tablet

Effects:

It reduces the negative influence of stress and excessive psysical burden on the organism. It supresses anxiety states and globally show calming effect. It contributes to the good peace of mind and physical state. It increases the imunity against physical tiredness, activates thinking and supports its concentration. It favourably influences defensiveness of the organism and it is suitable as an integral part of supporting treatment of the fatigue syndrom.

More...:

AnxoProtect tbl
Mgr. Katerina Horackova, Clinical department director

This preparative attenuates negative effects of stress and high mental endurance. It can help to keep down the anxiety and has soothing effect. It can conduce to good well-being. It helps to increase physical effort resistance, activates thinking and supports concentration. It has influence on organism striking power and is suitable as a supplement by fatigue syndrom treatment.
It contains extracts of Withania somnifera, Ocimum sanctum, Passiflora incarnata, Centella asiatica, Bacopa monnieri, Tinospora cordifolia, Asparagus racemosus, Emblica officinalis.

Withania somnifera
Many studies that investigate effects of Withania on nervous system has been made in last 5 years. Some of them investigated effect on dyskinesias and Parkinsonism. In year 2002 a study on a rat model of tardive dyskinesia has been made. Glycowithanolides were isolated and used for this study. Rats got haloperidol (1,5 mg/kg i.p.) once a day for 28 days for tardive dyskinesia induction. Glycowithanolides were administered in dose of 100 and 200 mg, p.o. concomitantly with haloperidol. Glycowithanolides inhibited the induction of neuroleptic tardive dyskinesia. The dyskinesia was also attenuated by vitamin E (because of its antioxidant properties), but no effect was observed after sodium valproat administration (GABA-mimetic antiepileptic agent). The results indicate that the antioxidant effect of glycowithanolides may be responsible for the prevention of haloperidol-induced tardive dyskinesia.1 In year 2006 another study has been made, in a rat model again, the dyskinesia has been induced by reserpine. After chronic treatment with root extract of Withania somnifera (4 weeks) the dyskinesia was significantly and dose dependently (50 and 100 mg/kg) reduced, reserpine-induced memory retention deficits were reversed. Chronic reserpine treatment significantly induced lipid peroxidation and decreased the glutathion levels in the brains. Chronic administration of extract significantly and dose dependently reduced the lipid peroxidation and restored the glutathion levels.2 The antioxidant effect is the main effect in protecting the neuronal injury in Parkinson disease, the effect is dose dependent.3 A study was made and investigated the protection effect on brain in a model of stroke in rats. The rats had been pretreated with extract of Withania somnifera for 15 and 30 days, than they were subjected to focal ischaemia. After 2 hours reperfusion was allowed. After 24 hours the animals were subjected to motor performance tests and were killed and investigated. The 30 days pretreatment was effective in contast to 15 days pretreatment. The antioxidant effect of extract may be responsible for protecting the neuronal injury caused by ischaemia by stroke.4
In year 2003 some studies investigating adaptogenic and anti-stress properties have been made. The adaptogenic activity was investigated against a rat model of chronic stress, rats got Withania somnifera (25 and 50mg/kg) p.o. or Panax ginseng (100mg/kg p.o.). The results show that Withania somnifera has (like Panax ginseng) significant antistress adaptogenic activity.5 Another study used isolated 1-oxo-5beta, 6beta-epoxy-witha-2-ene-27-ethoxy-olide for investigating. Stress-related indices were evaluated (creatine phosphokinase activity, lactate dehydrogenase activity, corticosterone levels, lipid peroxidation). There was a significant decrease in levels of all observed indices.6
A study investigating nootropic effect of Withania somnifera has been made in year 2001 in mice. A root extract was administered per os in doses of 50, 100 and 200 mg/kg. It improved retention of a passive avoidance task in a step-down paradigm, reversed the scopolamine-induced disruption of acquisition and retention and attenuated the amnesia.7

Ocimum sanctum
Some studies investigating antioxidant effect of Ocimum sanctum has been made in years 2004 and 2006. The first study investigated not only antioxidant, but also neuroprotective effects in rats. For this study a methanolic extract of Ocimum sanctum leaves was used. Occlusion of bilateral common carotid arteries had been made for 30 min, than followed 45 min reperfusion. Increase in lipid peroxidation and up-regulation of superoxid dismutase activity was observed. Ascorbic acid levels were unchanged. Pretreatment with extract (200 mg/kg/day for 7 days) prevented all these processes and stabilised levels of total sulfhydryl groups during reperfusion. Long-term cerebral hypoperfusion (induced by permanent occlusion for 15 days) caused behavioral and histopathological changes (cellular oedema, gliosis, perivascular inflammatory infiltrate). Treatment with extract (200 mg/kg/day for 15 days) significantly prevented these hypoperfusion-induced functional and structural changes.8 Another study from year 2006 has shown that Ocimum sanctum has antioxidant properties. Treatment of rabbits with Ocimum sanctum decreased lipid peroxidation and increased reduced glutathione content.9
A study from year 2006 has investigated nootropic effect of Ocimum sanctum. This study was made in mice, an aqueous extract was used. A control group was treated with piracetam. The extract ameliorated the amnesic effect of scopolamine, diazepam and aging induced memory deficits, decreased transfer latency and increased step down latency significantly.10
Two studies that has been made in year 2005 has investigated effect of Ocimum sanctum on changes by noise-stress. Both studies were made in rats and an ethanolic extract was used. In first study content of acetylcholine and activity of acetylcholinesterase in brain were observed. After exposure to noise stress significant reduction of acetylcholine content and increase of acetylcholinesterase activity were observed. Pretreatment with extract for 7 days prevented the changes.11 In second study other parameters were measured (norepinephrine, epinephrine, dopamine, serotonin). Administration of Ocimum sanctum extract normalized the processes in brain and controlled the alteration of neurotransmitter levels.12

Passiflora incarnata
Many studies investigating anxiolytic activity of Passiflora incarnata extracts were made. Benzoflavones isolated from this plant are responsible for the anxiolytic effect.13
A study from year 2002 has investigated suppression of alcohol-cessation-oriented hyper-anxiaty in mice. Bioactive benzoflavone moiety was administered to mice that were treated with ethylalcohol. The administration was chronic and acute. In both cases benzoflavones prevented significantly the expression of withdrawal effects of alcohol and there was a significant decrease in anxiety oriented behavior. The chronic administration of benzoflavones had better preventive effects than the single acute treatment.14

Centella asiatica
A study from year 2005 has investigated nootropic effect of aqueous extract. The study was made in mice, extract (200 mg/kg) was given from 15. day to 30. day after birth. Effect was observed from 31. day after birth to 6. month, behaviour, biochemical and histological parameters were evaluated. Performance was improved, but there was no significant effect on locomotor activity. Activity of acetylcholinesterase in hippocampus was increased and dendritic arborization of hippocampal CA3 neurons was also increased. Extract caused significant changes in neuronal morphology and improve of brain functions in young mice.15 Similar study from year 2005 has investigated influence of ethanolical extract and its fractions on nerve regeneration, concretely axonal regeneration. According to this study asiatic acid and probably some other compounds are responsible for this effect. Results show that extract can be useful in neuron damage.16
Bacopa monnieri
Effect of this plant or extract respectively on cognitive functions has been investigated in last years, many studies have been made. In year 2000 antioxidant effect of extract on brain cortex, striatum and hippocampus has been investigated in rats. The animals got the extract (5 and 10 mg/kg, bacoside A content 82%, p.o.) for 7, 14 and 21 days, the effect was compared with deprenyle (2 mg/kg, p.o.). Effect of extract was comparable to deprenyle, only by 14-days and 21-days administration there was no deprenyl effect in hippocampus in contrast to extract.17 In the same year another study has been made, this study has investigated effect on cognitive functions effected by phenytoin administration in mice. Phenytoin was administered in dose of 25 mg/kg p.o. for 14 days. Extract was administered in dose of 40 mg/kg for 7 days (it was the second week of phenytoin treatment). It was found that extract significantly decreases adverse effect of phenytoin on cognitive functions without influence on anticonvulsive effect of phenytoin.18 In year 2002 other studies have been made. One of them investigated antidepressive effect of this plant. The study was made in rats, the rats got methanolic extract in doses of 20 and 40 mg/kg, p.o. for 5 days. The effect was compared with imipramine that was administered in dose of 15 mg/kg, i.p. It was found that effect of extract is comparable with imipramine.19 In the next study effect of extract on human memory was investigated. 76 subjects in the age of 40 – 65 years were registered in this study, the study was double blind, randomised, in control group placebo was used. Different memory functions were tested and anxiety levels were measured. First tests were made before study beginning, second three months after beginning and third six weeks after end of study. The results showed significant effect of extract on new information keeping. Learning ability wasn´t induced, but there was a decrease of new information forgetting. Next observed parameters were not induced.20 In year 2003 a study investigating protective effect of extract directly on rat astrocytes culture. Cell damage rate caused by high concentration of NO (S-nitroso-N-acetyl-penicilamine was used) and cell protection rate by extract using was monitored. It was found that extract protects cells not only against damage by reactive compounds, but also against DNA damage dose dependently.21 In the same year another study has been made, in this study human fibroblasts were used. Free radicals scavenging activity and DNA damage protection of extract was investigated. Cell protection against damage and scavenging activity was found to be dose dependently.22

Tinospora cordifolia
This plant is an important antioxidant. Many studies investigating this effect were made. Two studies made in year 2002 have shown that extract and isolated polysaccharides can protect against radiation because of free radical scavenging.23,24 Tinospora has been investigated in year 2004 as an antioxidant useful by ischemic brain damage therapy. The study showed high antioxidant properties.25 Next studies showed high effect on antioxidant enzymes in liver and kidney.26,27

Asparagus racemosus
This plant is an antioxidant, too. A study investigating attenuation of oxidative damage in mice brain by extract of Asparagus. Hippocampal and striatal damage was caused by kainic acid. The study showed that the extract increase glutathionreductase activity and glutathione content and decreased membranal lipid peroxidation.28
A study investigating antiulcer and antioxidant activity of Asparagus racemosus and Withania somnifera has been made in year 2005. Results showed that both extracts have antiulcer activity, Asparagus is more effective against indomethacine-induced ulcers, Withania against stress-induced ulcers. Both extracts showed high antioxidant activity.29

Emblica officinalis
This plant is well known as a strong antioxidant. Many studies investigated this effect. Hepatoprotective activity of tannins from this plant was investigated in rats. The rats became a fraction of fresh juice in dosis 10, 20 and 50 mg/kg body weight for 10 days. After proposal of iron dose (30 mg/kg body weight) there was observed hepatoprotective effect analogic to the silymarin effect.30
The antioxidant properties on some next organs were investigated. One study from year 2004 has shown that Emblica can protect myocardium against oxidative damage caused by ischaemic-reperfusion injury, this study has been made in rats. The results indicate that chronic Emblica officinalis administration causes myocardial adaptation by augmenting endogenous antioxidants and protects rat hearts from oxidative stress associated with ischemic-reperfusion injury.31
Alcoholical extracts have gastroprotective effects. Two studies were made, the first with methanolical extract, the second with ethanolical extract. Both studies were made in rats. Methanolical extract was given to the rats, by that the gastric ulcerations were made by various effects (aspirin, ethanol, stress). After treatment of extract (dose 20 mg/kg body weight) the rats were after 5-10 days healthy, by lower doses a gastroprotective effect was observed.32 Ethanolical extract was given in dosis 250 and 500 mg/kg body weight in the same indications. All types of ulcerations were cured, and an antisecretion effect of extract was observed.33

Sources:
1. Bhattacharya, S.K., Bhattacharya, D., Sairam, K., Ghosal, S.: Effect of Withania somnifera glycowithanolides on a rat model of tardive dyskinesia. Phytomedicine, 2002, 9(2), 167-70.
2. Naidu, P.S., Singh, A., Kulkarni, S.K.: Effect of Withania somnifera root extract on reserpine-induced orofacial dyskinesia and cognitive dysfunction. Phytother. Res., 2006, 20(2), 140-6.
3. Ahmad, M., Saleem, S., Ahmad, A.S., Ansari, M.A., Yousuf, S., Hoda, M.N., Islam, F.: Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats. Hum. Exp. Toxicol., 2005, 24(3), 137-47.
4. Chaudhary, G., Sharma, U., Jagannathan, N.R., Gupta, Y.K.: Evaluation of Withania somnifera in a middle cerebral artery occlusion model of stroke in rats. Clin. Exp. Pharmacol. Physiol., 2003, 30(5-6), 399-404.
5. Bhattacharya, S.K., Muruganandam, A.V.: Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress. Pharmacol. Biochem. Behav., 2003, 75(3), 547-55.
6. Kaur, P., Sharma, M., Mathur, S., Tiwari, M., Divekar, H.M., Kumar, R., Srivastava, K.K., Chandra, R.: Effect of 1-oxo-5beta, 6beta-epoxy-witha-2-ene-27-ethoxy-olide isolated from the roots of Withania somnifera on stress indices in Wistar rats. J. Altern. Complement. Med., 2003, 9(6), 897-907.
7. Dhuley, J.N.: Nootropic-like effect of ashwagandha (Withania somnifera L.) in mice. Phytother. Res., 2001, 15(6), 524-8.
8. Yanpallewar, S.U., Rai, S., Kumar, M., Acharya, S.B.: Evaluation of antioxidant and neuroprotective effect of Ocimum sanctum on transient cerebral ischemia and long-term cerebral hypoperfusion. Pharmacol. Biochem. Behav., 2004, 79(1), 155-64.
9. Gupta, S., Mediratta, P.K., Singh, S., Sharma, K.K., Shukla, R.: Antidiabetic, antihypercholesterolaemic and antioxidant effect of Ocimum sanctum (Linn) seed oil. Indian J. Exp. Biol., 2006, 44(4), 300-4.
10. Joshi, H., Parle, M.: Evaluation of nootropic potential of Ocimum sanctum Linn. in mice. Indian J. Exp. Biol., 2006, 44(2), 133-6.
11. Sembulingam, K., Sembulingam, P., Namasivayam, A.: Effect of Ocimum sanctum Linn on the changes in central cholinergic system induced by acute noise stress. J. Ethnopharmacol., 2005, 96(3), 477-82.
12. Ravindran, R., Rathinasamy, S.D., Samson, J., Senthilvelan, M.: Noise-stress-induced brain neurotransmitter changes and the effect of Ocimum sanctum (Linn) treatment in albino rats. J. Pharmacol. Sci., 2005, 98(4), 354-60.
13. Dhawan, K., Kumar, S., Sharma, A.: Anti-anxiety studies on extracts of Passiflora incarnata Linneaus. J. Ethnopharmacol., 2001, 78(2-3), 165-70.
14. Dhawan, K., Kumar, S., Sharma, A.: Suppression of alcohol-cessation-oriented hyper-anxiety by the benzoflavone moiety of Passiflora incarnata Linneaus in mice. J. Ethnopharmacol., 2002, 81(2), 239-44.
15. Rao, S.B., Chetana, M., Uma Devi, P.: Centella asiatica treatment during postnatal period enhances learning and memory in mice. Physiol Behav., 2005, 86(4), 449-57.
16. Soumyanath, A., Zhong, Y.P., Gold, S.A., Yu, X., Koop, D.R., Bourdette, D., Gold, B.G.: Centella asiatica accelerates nerve regeneration upon oral administration and contains multiple active fractions increasing neurite elongation in-vitro. J. Pharm. Pharmacol., 2005, 57(9), 1221-9.
17. Bhattacharya, S.K., Bhattacharya, A., Kumar, A., Ghosal, S.: Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. Phytother. Res., 2000, 14, 174-179.
18. Vohora, D., Pal, S.N., Pillai, K.K.: Protection from phenytoin-induced cognitive deficit by Bacopa monniera, a reputed Indian nootropic plant. J. Ethnopharmacol., 2000, 71, 383-390.
19. Sairam, K., Dorababu, M., Goel, R.K., Bhattacharya, S.K.: Antidepressant activity of standardized extract of Bacopa monniera in experimental models of depression in rats. Phytomedicine, 2002, 9, 207-211.
20. Roodenrys, S., Booth, D., Bulzomi, S., Phipps, A., Micallef, C., Smoker, J.: Chronic effects of Brahmi (Bacopa monnieri) on human memory. Neuropsychopharmacology, 2002, 27, 279-281.
21. Russo, A., Borrelli, F., Campisi, A., Acquivava, R., Raciti, G., Vanella, A.: Nitric oxide-related toxicity in cultured astrocytes: effect of Bacopa monniera. Life Sciences, 2003, 73, 1517-1526.
22. Russo, A., Izzo, A.A., Borrelli, F., Renis, M., Vanella, A.: Free radical scavengig capacity and protective effect of Bacopa monniera L. on DNA damage. Phytother. Res., 2003, 17, 870-875.
23. Subramanian, M., Chintalwar, G.J., Chattopadhyay, S.: Antioxidant properties of a Tinospora cordifolia polysaccharide against iron-mediated lipid damage and gamma-ray induced protein damage. Redox. Rep., 2002,7(3), 137-43.
24. Goel, H.C., Prem Kumar, I., Rana, S.V.: Free radical scavenging and metal chelation by Tinospora cordifolia, a possible role in radioprotection. Indian J. Exp. Biol., 2002, 40(6), 727-34.
25. Rawal, A., Muddeshwar, M., Biswas, S.: Effect of Rubia cordifolia, Fagonia cretica linn, and Tinospora cordifolia on free radical generation and lipid peroxidation during oxygen-glucose deprivation in rat hippocampal slices. Biochem. Biophys. Res. Commun., 2004, 324(2), 588-96.
26. Prince, P.S., Padmanabhan, M., Menon, V.P.: Restoration of antioxidant defence by ethanolic Tinospora cordifolia root extract in alloxan-induced diabetic liver and kidney. Phytother. Res., 2004, 18(9),785-7.
27. Singh, R.P., Banerjee, S., Kumar, P.V., Raveesha, K.A., Rao, A.R.: Tinospora cordifolia induces enzymes of carcinogen/drug metabolism and antioxidant system, and inhibits lipid peroxidation in mice. Phytomedicine, 2006, 13(1-2),74-84.
28. Parihar, M.S., Hemnani, T.: Experimental excitotoxicity provokes oxidative damage in mice brain and attenuation by extract of Asparagus racemosus. J. Neural. Transm., 2004, 111(1),1-12.
29. Bhatnagar, M., Sisodia, S.S., Bhatnagar, R.: Antiulcer and Antioxidant Activity of Asparagus racemosus WILLD and Withania somnifera DUNAL in Rats. Ann. N. Y. Acad. Sci., 2005, 1056, 261-78.
30. Bhattacharya A., Kumar M., Ghosal S., Bhattacharya S.K.: Effect of bioactive tannoid principles of Emblica officinalis on iron-induced hepatic toxicity in rats. Phytomedicine, 2000, 7, 173-175.
31. Rajak, S., Banerjee, S.K., Sood, S., Dinda, A.K., Gupta, Y.K., Gupta, S.K., Maulik, S.K.: Emblica officinalis causes myocardial adaptation and protects against oxidative stress in ischemic-reperfusion injury in rats. Phytother. Res., 2004, 18(1),54-60.
32. Sairam K., Rao Ch.V., Babu M.D., Kumar K.V., Agrawal V.K., K Goel R.K.: Antiulcerogenic effect of methanolic extract of Emblica officinalis: an experimental study. J. Ethnopharmacol., 2002, 82, 1-9.
33. Al-Rehaily A.J., Al-Howiriny T.A., Al-Sohaibani M.O., Rafatullah S.: Gastroprotective effects of Amla (Emblica officinalis) on in vivo models in rats. Phytomedicine, 2002, 9, 515-522.

up